Effects of Adiponectin on Glucose Metabolism in the Hepatopancreas of Grass Carp (Ctenopharyngodon idella)

Adiponectin plays critical roles in the regulation of energy metabolism mammals. However, its fish remain to be evaluated. In this study, we examined tissue distribution characteristics two adiponectin genes, namely, adipoqa and adipoqb, at transcriptional level, detected changes their mRNA expressions during oral glucose tolerance test (OGTT). Furthermore, prepared recombinant proteins grass carp A B studied hepatopancreas vivo. The results showed that was mainly expressed heart carp, followed by red muscle, white adipose tissue, while adipoqb highly muscle. levels muscle were significantly decreased 1 h post OGTT, also suppressed 3 h, whereas elevated 12 after load. Intraperitoneal injection or reduced basal serum caused an increase glycogen content hepatopancreas. upregulated expression enzyme genes related glycolysis (gk pk) synthesis (gys) hepatopancreas, promoted pk gys, as well g6pase glycogenetic pathway. Besides, have opposite regulatory effects on glut2 expression. These suggested glycolysis, gluconeogenesis, synthesis, increased blood they may antagonistic mechanism regulating uptake.